| Children |
| | 1. Middleton, Rev John, b. 31 Jan 1811, Johnstown, New York, United States d. 14 Sep 1872, Stanton, Illinois, United States (Age 61 years) |
| | 2. Middleton, Cathrine Margaret d. 1852 |
| | 3. Middleton, Euphame, b. 29 Apr 1801, Johnstown, New York d. 15 May 1811, Johnstown, New York (Age 10 years) |
| | 4. Middleton, George Warren, b. 7 Jun 1805 d. 1866 (Age 60 years) |
| | 5. Middleton, Joseph, b. 4 Nov 1812, Johnstown, New York d. 10 Apr 1891, Ontario, New York (Age 78 years) |
| | 6. Middleton, William Henry, b. 9 Mar 1816, New York, United States d. 23 Jun 1842, New York, United States (Age 26 years) |
| | 7. Middleton, James M, b. 15 Aug 1819, New York, United States d. 19 Apr 1901, Ohio, United States (Age 81 years) |
| | 8. Middleton, Jane Elizabeth, b. 25 Jun 1821 d. 25 Jan 1889 (Age 67 years) |
| | 9. Middleton, Joseph, b. 1820, Ontario, Wayne, New York d. Yes, date unknown |
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